Inhaled NO improves brain damage by collateral recruitment in a neonatal stroke model
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چکیده
Supplemental Methods Neonatal ischemia-reperfusion. All experiments complied with ethical guidelines of Robert Debré Hospital Research Council Review Board (A75-19-01), INSERM and the ARRIVE guidelines (http://www.nc3rs.org/ARRIVE). Ischemia was induced in Wistar P7 rat pups (1618 g; Janvier, Le Genest St-Isle, France; both sexes) and was adapted to isoflurane anesthesia . Briefly, thermoregulated (37.0±0.5C°) and anesthetized pups [1% isoflurane in O2/N2O (1:3)] were exposed to left middle cerebral artery electocoagulation (MCAo) combined with a transient (50 min) and concomitant occlusion of both common carotid arteries (CCA) by using vascular clips (18055-04, Fine Science Tools, Heidelberg, Germany). Carotid blood-flow restoration was verified with the aid of a microscope. During ischemia and recovery the pups were placed in a humidified incubator at 32-34°C. After recovery, pups were transferred to their mothers.
منابع مشابه
Inhaled nitric oxide reduces brain damage by collateral recruitment in a neonatal stroke model.
BACKGROUND AND PURPOSE We recently demonstrated that endogenous nitric oxide (NO) modulates collateral blood flow in a neonatal stroke model in rats. The inhalation of NO (iNO) has been found to be neuroprotective after ischemic brain damage in adults. Our objective was to examine whether iNO could modify cerebral blood flow during ischemia-reperfusion and reduce lesions in the developing brain...
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